Low input DNA size selection on the Pippin Prep System using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kit

With the advent of next-generation sequencing (NGS), library preparation has become a much more streamlined process utilizing smaller amounts of input genomic DNA, thus allowing for more narrow fragmentation profi les. This Application Note demonstrates the usefulness of the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA kit in complementing the Pippin Prep automated size selection workfl ow from Sage Science, Inc. The 2100 Bioanalyzer is capable of analyzing the size distribution of fragmented input DNA, permitting users to specify the appropriate size selection range on the Pippin Prep system. Together, these technologies enable fi ne control and increased effi ciency with the critical fi rst step of library generation. Author Chris Boles, Ph.D Sage Science, Inc. Beverly, MA USA